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KMID : 0923620140140010054
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Immune Network
2014 Volume.14 No. 1 p.54 ~ p.65
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Molecular Characterization of Neurally Differentiated Human Bone Marrow-derived Clonal Mesenchymal Stem Cells
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Yi Tac-Ghee
Lee Hyun-Joo
Cho Yun-Kyoung
Jeon Myung-Shin
Song Sun-U.
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Abstract
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Bone marrow-derived mesenchymal stem cells (MSCs) are multipotent, with the ability to differentiate into different cell types. Additionally, the immunomodulatory activity of MSCs can downregulate inflammatory responses. The use of MSCs to repair injured tissues and treat inflammation, including in neuroimmune diseases, has been extensively explored. Although MSCs have emerged as a promising resource for the treatment of neuroimmune diseases, attempts to define the molecular properties of MSCs have been limited by the heterogeneity of MSC populations. We recently developed a new method, the subfractionation culturing method, to iso-late homogeneous human clonal MSCs (hcMSCs). The hcMSCs were able to differentiate into fat, cartilage, bone, neuroglia, and liver cell types. In this study, to better under-stand the properties of neurally differentiated MSCs, gene expression in highly homogeneous hcMSCs was analyzed. Neural differentiation of hcMSCs was induced for 14 days. Thereafter, RNA and genomic DNA was isolated and sub-jected to microarray analysis and DNA methylation array analysis, respectively. We correlated the transcriptome of hcMSCs during neural differentiation with the DNA methyl-ation status. Here, we describe and discuss the gene ex-pression profile of neurally differentiated hcMSCs. These findings will expand our understanding of the molecular prop-erties of MSCs and contribute to the development of cell therapy for neuroimmune diseases.
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KEYWORD
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hcMSC, Mesenchymal stem cell, Neural differentiation, Microarray, Methylation, Subfractionation culturing method
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